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		<doi>10.3390/ijms17050672</doi>
		<issn>1422-0067</issn>
		<label>lattes: 4809959472520732 2 ZimbardiCCAMRAJF:2016:PoApDy</label>
		<citationkey>ZimbardiCCAMRAJF:2016:PoApDy</citationkey>
		<title>A high redox potential laccase from pycnoporus sanguineus RP15: potential application for dye decolorization</title>
		<year>2016</year>
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		<author>Zimbardi, Ana,</author>
		<author>Camargo, Priscila de Fátima,</author>
		<author>Carli, Sibeli,</author>
		<author>Aquino Neto, Sidney,</author>
		<author>Meleiro, Luana P.,</author>
		<author>Rosa, José C.,</author>
		<author>Andrade, Adalgisa R. de,</author>
		<author>Jorge, João A.,</author>
		<author>Furriel, Rosa P. M.,</author>
		<group></group>
		<group>LCP-CTE-INPE-MCTI-GOV-BR</group>
		<affiliation>Universidade de São Paulo (USP)</affiliation>
		<affiliation>Instituto Nacional de Pesquisas Espaciais (INPE)</affiliation>
		<affiliation>Universidade de São Paulo (USP)</affiliation>
		<affiliation>Universidade de São Paulo (USP)</affiliation>
		<affiliation>Universidade de São Paulo (USP)</affiliation>
		<affiliation>Universidade de São Paulo (USP)</affiliation>
		<affiliation>Universidade de São Paulo (USP)</affiliation>
		<affiliation>Universidade de São Paulo (USP)</affiliation>
		<affiliation>Universidade de São Paulo (USP)</affiliation>
		<electronicmailaddress>luccilatorre@usp.br</electronicmailaddress>
		<electronicmailaddress>priscila@lcp.inpe.br</electronicmailaddress>
		<electronicmailaddress>sibelicarli@usp.br</electronicmailaddress>
		<electronicmailaddress>netaoaquino@gmail.com</electronicmailaddress>
		<electronicmailaddress>luanapm@usp.br</electronicmailaddress>
		<electronicmailaddress>jcrosa@fmrp.usp.br</electronicmailaddress>
		<electronicmailaddress>ardandra@ffclrp.usp.br</electronicmailaddress>
		<electronicmailaddress>joajorge@ffclrp.usp.br</electronicmailaddress>
		<journal>International Journal of Molecular Sciences</journal>
		<volume>17</volume>
		<number>5</number>
		<pages>672</pages>
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		<keywords>Pycnoporus sanguineus, solid state fermentation, response surface methodology, laccase, purification, characterization, decolorization, remazol brilliant blue R, Reactive blue 4, bromophenol blue.</keywords>
		<abstract>Laccase production by Pycnoporus sanguineus RP15 grown in wheat bran and corncob under solid-state fermentation was optimized by response surface methodology using a Central Composite Rotational Design. A laccase (Lacps1) was purified and characterized and the potential of the pure Lacps1 and the crude culture extract for synthetic dye decolorization was evaluated. At optimal conditions (eight days, 26 &#733;C, 18% (w/w) milled corncob, 0.8% (w/w) NH4Cl and 50 mmol¨ L ´1 CuSO4, initial moisture 4.1 mL¨ g ´1 ), the laccase activity reached 138.6 &#728; 13.2 U¨ g ´1 . Lacps1 was a monomeric glycoprotein (67 kDa, 24% carbohydrate). Optimum pH and temperature for the oxidation of 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) were 4.4 and 74.4 &#733;C, respectively. Lacps1 was stable at pH 3.08.0, and after two hours at 5560 &#733;C, presenting high redox potential (0.747 V vs. NHE). ABTS was oxidized with an apparent affinity constant of 147.0 &#728; 6.4 µmol¨ L ´1 , maximum velocity of 413.4 &#728; 21.2 U¨ mg´1 and catalytic efficiency of 3140.1 &#728; 149.6 L¨ mmol´1 ¨ s ´1 . The maximum decolorization percentages of bromophenol blue (BPB), remazol brilliant blue R and reactive blue 4 (RB4), at 25 or 40 &#733;C without redox mediators, reached 90%, 80% and 60%, respectively, using either pure Lacps1 or the crude extract. This is the first study of the decolorization of BPB and RB4 by a P. sanguineus laccase. The data suggested good potential for treatment of industrial dye-containing effluents.</abstract>
		<area>COMB</area>
		<language>en</language>
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